A Thermostable β-Glucuronidase Obtained by Directed Evolution as a Reporter Gene in Transgenic Plants

A β-glucuronidase variant, GUS-TR3337, that was obtained by directed evolution exhibited higher thermostability than the wild-type enzyme, GUS-WT. In this study, the utility of GUS-TR337 as an improved reporter was evaluated. The corresponding gus-tr3337 and gus-wt genes were independently cloned in...

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Detalles Bibliográficos
Autores principales: Xiong, Ai-Sheng, Peng, Ri-He, Zhuang, Jing, Chen, Jian-Min, Zhang, Bin, Zhang, Jian, Yao, Quan-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212524/
https://www.ncbi.nlm.nih.gov/pubmed/22096498
http://dx.doi.org/10.1371/journal.pone.0026773

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212524/
https://www.ncbi.nlm.nih.gov/pubmed/22096498
http://dx.doi.org/10.1371/journal.pone.0026773

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