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An automated cell-counting algorithm for fluorescently-stained cells in migration assays
A cell-counting algorithm, developed in Matlab(®), was created to efficiently count migrated fluorescently-stained cells on membranes from migration assays. At each concentration of cells used (10,000, and 100,000 cells), images were acquired at 2.5 ×, 5 ×, and 10 × objective magnifications. Automat...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3214125/ https://www.ncbi.nlm.nih.gov/pubmed/22011343 http://dx.doi.org/10.1186/1480-9222-13-9 |