Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination

We describe a site-specific recombination-based tandem assembly (SSRTA) method for reconstruction of biological parts in synthetic biology. The system was catalyzed by Streptomyces phage φBT1 integrase, which belongs to the large serine recombinase subfamily. This one-step approach was efficient and...

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Detalles Bibliográficos
Autores principales: Zhang, Lin, Zhao, Guoping, Ding, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216622/
https://www.ncbi.nlm.nih.gov/pubmed/22355658
http://dx.doi.org/10.1038/srep00141
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author Zhang, Lin
Zhao, Guoping
Ding, Xiaoming
author_facet Zhang, Lin
Zhao, Guoping
Ding, Xiaoming
author_sort Zhang, Lin
collection PubMed
description We describe a site-specific recombination-based tandem assembly (SSRTA) method for reconstruction of biological parts in synthetic biology. The system was catalyzed by Streptomyces phage φBT1 integrase, which belongs to the large serine recombinase subfamily. This one-step approach was efficient and accurate, and able to join multiple DNA molecules in vitro in a defined order. Thus, it could have applications in constructing metabolic pathways and genetic networks.
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spelling pubmed-32166222011-12-22 Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination Zhang, Lin Zhao, Guoping Ding, Xiaoming Sci Rep Article We describe a site-specific recombination-based tandem assembly (SSRTA) method for reconstruction of biological parts in synthetic biology. The system was catalyzed by Streptomyces phage φBT1 integrase, which belongs to the large serine recombinase subfamily. This one-step approach was efficient and accurate, and able to join multiple DNA molecules in vitro in a defined order. Thus, it could have applications in constructing metabolic pathways and genetic networks. Nature Publishing Group 2011-11-03 /pmc/articles/PMC3216622/ /pubmed/22355658 http://dx.doi.org/10.1038/srep00141 Text en Copyright © 2011, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Zhang, Lin
Zhao, Guoping
Ding, Xiaoming
Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
title Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
title_full Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
title_fullStr Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
title_full_unstemmed Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
title_short Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
title_sort tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216622/
https://www.ncbi.nlm.nih.gov/pubmed/22355658
http://dx.doi.org/10.1038/srep00141
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AT zhaoguoping tandemassemblyoftheepothilonebiosyntheticgeneclusterbyinvitrositespecificrecombination
AT dingxiaoming tandemassemblyoftheepothilonebiosyntheticgeneclusterbyinvitrositespecificrecombination

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