Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination

We describe a site-specific recombination-based tandem assembly (SSRTA) method for reconstruction of biological parts in synthetic biology. The system was catalyzed by Streptomyces phage φBT1 integrase, which belongs to the large serine recombinase subfamily. This one-step approach was efficient and...

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Detalles Bibliográficos
Autores principales: Zhang, Lin, Zhao, Guoping, Ding, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216622/
https://www.ncbi.nlm.nih.gov/pubmed/22355658
http://dx.doi.org/10.1038/srep00141

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216622/
https://www.ncbi.nlm.nih.gov/pubmed/22355658
http://dx.doi.org/10.1038/srep00141

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