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Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination
We describe a site-specific recombination-based tandem assembly (SSRTA) method for reconstruction of biological parts in synthetic biology. The system was catalyzed by Streptomyces phage φBT1 integrase, which belongs to the large serine recombinase subfamily. This one-step approach was efficient and...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216622/ https://www.ncbi.nlm.nih.gov/pubmed/22355658 http://dx.doi.org/10.1038/srep00141 |