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Quantitative Co-Expression of Proteins at the Single Cell Level – Application to a Multimeric FRET Sensor

BACKGROUND: Co-expression of proteins is generally achieved by introducing two (or more) independent plasmids into cells, each driving the expression of a different protein of interest. However, the relative expression levels may vary strongly between individual cells and cannot be controlled. Ideal...

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Detalles Bibliográficos
Autores principales: Goedhart, Joachim, van Weeren, Laura, Adjobo-Hermans, Merel J.W., Elzenaar, Ies, Hink, Mark A., Gadella, Theodorus W.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219669/
https://www.ncbi.nlm.nih.gov/pubmed/22114669
http://dx.doi.org/10.1371/journal.pone.0027321