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False negative results from using common PCR reagents

BACKGROUND: The sensitivity of the PCR reaction makes it ideal for use when identifying potentially novel viral infections in human disease. Unfortunately, this same sensitivity also leaves this popular technique open to potential contamination with previously amplified PCR products, or "carry-...

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Detalles Bibliográficos
Autores principales: Bacich, Dean J, Sobek, Kathryn M, Cummings, Jessica L, Atwood, Allison A, O'Keefe, Denise S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219698/
https://www.ncbi.nlm.nih.gov/pubmed/22032271
http://dx.doi.org/10.1186/1756-0500-4-457