Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run

Optical mapping of bacterial chromosomes provides an unambiguous low-resolution sequence scaffold of the entire chromosome. In comparison to some techniques, such as pulse field gel electrophoresis, cost and throughput limit the application of this technique outside of genome finishing. We have demo...

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Detalles Bibliográficos
Autores principales: Riley, Matthew C., Lee, James Eric, Lesho, Emil, Kirkup, Benjamin C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3221658/
https://www.ncbi.nlm.nih.gov/pubmed/22132087
http://dx.doi.org/10.1371/journal.pone.0027085
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author Riley, Matthew C.
Lee, James Eric
Lesho, Emil
Kirkup, Benjamin C.
author_facet Riley, Matthew C.
Lee, James Eric
Lesho, Emil
Kirkup, Benjamin C.
author_sort Riley, Matthew C.
collection PubMed
description Optical mapping of bacterial chromosomes provides an unambiguous low-resolution sequence scaffold of the entire chromosome. In comparison to some techniques, such as pulse field gel electrophoresis, cost and throughput limit the application of this technique outside of genome finishing. We have demonstrated the production of multiple bacterial maps using a single set of consumables; this significantly reduces the time and expense of map production.
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spelling pubmed-32216582011-11-30 Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run Riley, Matthew C. Lee, James Eric Lesho, Emil Kirkup, Benjamin C. PLoS One Research Article Optical mapping of bacterial chromosomes provides an unambiguous low-resolution sequence scaffold of the entire chromosome. In comparison to some techniques, such as pulse field gel electrophoresis, cost and throughput limit the application of this technique outside of genome finishing. We have demonstrated the production of multiple bacterial maps using a single set of consumables; this significantly reduces the time and expense of map production. Public Library of Science 2011-11-21 /pmc/articles/PMC3221658/ /pubmed/22132087 http://dx.doi.org/10.1371/journal.pone.0027085 Text en This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Riley, Matthew C.
Lee, James Eric
Lesho, Emil
Kirkup, Benjamin C.
Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run
title Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run
title_full Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run
title_fullStr Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run
title_full_unstemmed Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run
title_short Optically Mapping Multiple Bacterial Genomes Simultaneously in a Single Run
title_sort optically mapping multiple bacterial genomes simultaneously in a single run
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3221658/
https://www.ncbi.nlm.nih.gov/pubmed/22132087
http://dx.doi.org/10.1371/journal.pone.0027085
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