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State-of-the-Art Automated Patch Clamp Devices: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening

Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch cla...

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Autores principales: Stoelzle, Sonja, Obergrussberger, Alison, Brüggemann, Andrea, Haarmann, Claudia, George, Michael, Kettenhofen, Ralf, Fertig, Niels
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Research Foundation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3223389/
https://www.ncbi.nlm.nih.gov/pubmed/22131976
http://dx.doi.org/10.3389/fphar.2011.00076
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author Stoelzle, Sonja
Obergrussberger, Alison
Brüggemann, Andrea
Haarmann, Claudia
George, Michael
Kettenhofen, Ralf
Fertig, Niels
author_facet Stoelzle, Sonja
Obergrussberger, Alison
Brüggemann, Andrea
Haarmann, Claudia
George, Michael
Kettenhofen, Ralf
Fertig, Niels
author_sort Stoelzle, Sonja
collection PubMed
description Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch clamp (APC) systems with high-throughput capabilities. These systems are now available for drug screening, but there are limitations in the application range. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. The addition of well controlled and fast solution exchange, temperature control and the availability of the current clamp mode are required to analyze standard cell lines and excitable cells such as stem cell-derived cardiomyocytes in a more physiologically relevant environment. Here we describe two systems with different areas of applications that meet the needs of drug discovery researchers and basic researchers alike. The here utilized medium throughput APC device is a planar patch clamp system capable of recording up to eight cells simultaneously. Features such as temperature control and recordings in the current clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage clamp and current clamp modes with the view to finding new drug candidates and safety testing methods in a more physiologically relevant environment. The high-throughput system used here is a planar patch clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data provided reveals the suitability and relevance of both APC platforms for drug discovery, ion channel research, and safety testing.
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spelling pubmed-32233892011-11-30 State-of-the-Art Automated Patch Clamp Devices: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening Stoelzle, Sonja Obergrussberger, Alison Brüggemann, Andrea Haarmann, Claudia George, Michael Kettenhofen, Ralf Fertig, Niels Front Pharmacol Pharmacology Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch clamp (APC) systems with high-throughput capabilities. These systems are now available for drug screening, but there are limitations in the application range. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. The addition of well controlled and fast solution exchange, temperature control and the availability of the current clamp mode are required to analyze standard cell lines and excitable cells such as stem cell-derived cardiomyocytes in a more physiologically relevant environment. Here we describe two systems with different areas of applications that meet the needs of drug discovery researchers and basic researchers alike. The here utilized medium throughput APC device is a planar patch clamp system capable of recording up to eight cells simultaneously. Features such as temperature control and recordings in the current clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage clamp and current clamp modes with the view to finding new drug candidates and safety testing methods in a more physiologically relevant environment. The high-throughput system used here is a planar patch clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data provided reveals the suitability and relevance of both APC platforms for drug discovery, ion channel research, and safety testing. Frontiers Research Foundation 2011-11-24 /pmc/articles/PMC3223389/ /pubmed/22131976 http://dx.doi.org/10.3389/fphar.2011.00076 Text en Copyright © 2011 Stoelzle, Obergrussberger, Brüggemann, Haarmann, George, Kettenhofen and Fertig. http://www.frontiersin.org/licenseagreement This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.
spellingShingle Pharmacology
Stoelzle, Sonja
Obergrussberger, Alison
Brüggemann, Andrea
Haarmann, Claudia
George, Michael
Kettenhofen, Ralf
Fertig, Niels
State-of-the-Art Automated Patch Clamp Devices: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
title State-of-the-Art Automated Patch Clamp Devices: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
title_full State-of-the-Art Automated Patch Clamp Devices: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
title_fullStr State-of-the-Art Automated Patch Clamp Devices: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
title_full_unstemmed State-of-the-Art Automated Patch Clamp Devices: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
title_short State-of-the-Art Automated Patch Clamp Devices: Heat Activation, Action Potentials, and High Throughput in Ion Channel Screening
title_sort state-of-the-art automated patch clamp devices: heat activation, action potentials, and high throughput in ion channel screening
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3223389/
https://www.ncbi.nlm.nih.gov/pubmed/22131976
http://dx.doi.org/10.3389/fphar.2011.00076
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