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A bacteria colony-based screen for optimal linker combinations in genetically encoded biosensors

BACKGROUND: Fluorescent protein (FP)-based biosensors based on the principle of intramolecular Förster resonance energy transfer (FRET) enable the visualization of a variety of biochemical events in living cells. The construction of these biosensors requires the genetic insertion of a judiciously ch...

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Detalles Bibliográficos
Autores principales: Ibraheem, Andreas, Yap, Hongkin, Ding, Yidan, Campbell, Robert E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3225322/
https://www.ncbi.nlm.nih.gov/pubmed/22074568
http://dx.doi.org/10.1186/1472-6750-11-105