Cargando…

Systematic Comparison of Three Methods for Fragmentation of Long-Range PCR Products for Next Generation Sequencing

Next Generation Sequencing (NGS) technologies are gaining importance in the routine clinical diagnostic setting. It is thus desirable to simplify the workflow for high-throughput diagnostics. Fragmentation of DNA is a crucial step for preparation of template libraries and various methods are current...

Descripción completa

Detalles Bibliográficos
Autores principales:	Knierim, Ellen, Lucke, Barbara, Schwarz, Jana Marie, Schuelke, Markus, Seelow, Dominik
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3227650/ https://www.ncbi.nlm.nih.gov/pubmed/22140562 http://dx.doi.org/10.1371/journal.pone.0028240

Ejemplares similares

MutationDistiller: user-driven identification of pathogenic DNA variants
por: Hombach, Daniela, et al.
Publicado: (2019)

GeneDistiller—Distilling Candidate Genes from Linkage Intervals
por: Seelow, Dominik, et al.
Publicado: (2008)

HomozygosityMapper2012—bridging the gap between homozygosity mapping and deep sequencing
por: Seelow, Dominik, et al.
Publicado: (2012)

A systematic, large-scale comparison of transcription factor binding site models
por: Hombach, Daniela, et al.
Publicado: (2016)

Erratum to: A systematic, large-scale comparison of transcription factor binding site models
por: Hombach, Daniela, et al.
Publicado: (2016)

RegulationSpotter: annotation and interpretation of extratranscriptic DNA variants
por: Schwarz, Jana Marie, et al.
Publicado: (2019)

MutationTaster2021
por: Steinhaus, Robin, et al.
Publicado: (2021)

HomozygosityMapper—an interactive approach to homozygosity mapping
por: Seelow, Dominik, et al.
Publicado: (2009)

Long-range PCR in next-generation sequencing: comparison of six enzymes and evaluation on the MiSeq sequencer
por: Jia, Haiying, et al.
Publicado: (2014)

Next Generation Sequencing Based Multiplex Long-Range PCR for Routine Genotyping of Autoinflammatory Disorders
por: Guzel, Ferhat, et al.
Publicado: (2021)

Long-range PCR in next generation sequencing: A low cost approach for large & complex genes
por: Chowdhary, Madhumita Roy, et al.
Publicado: (2023)

Non-random DNA fragmentation in next-generation sequencing
por: Poptsova, Maria S., et al.
Publicado: (2014)

FragIdent – Automatic identification and characterisation of cDNA-fragments
por: Seelow, Dominik, et al.
Publicado: (2009)

Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing
por: Ignatov, Konstantin B., et al.
Publicado: (2019)

Microsatellite instability in prostate cancer by PCR or next-generation sequencing
por: Hempelmann, Jennifer A., et al.
Publicado: (2018)

Fatal Cardiac Arrhythmia and Long-QT Syndrome in a New Form of Congenital Generalized Lipodystrophy with Muscle Rippling (CGL4) Due to PTRF-CAVIN Mutations
por: Rajab, Anna, et al.
Publicado: (2010)

MESA: automated assessment of synthetic DNA fragments and simulation of DNA synthesis, storage, sequencing and PCR errors
por: Schwarz, Michael, et al.
Publicado: (2020)

Next-generation sequencing using microfluidic PCR enrichment for molecular autopsy
por: Raju, Hariharan, et al.
Publicado: (2019)

When to use next generation sequencing or diagnostic PCR in diet analyses
por: Rennstam Rubbmark, Oskar, et al.
Publicado: (2019)

A New Mechanism in THRA Resistance: The First Disease-Associated Variant Leading to an Increased Inhibitory Function of THRA2
por: Paisdzior, Sarah, et al.
Publicado: (2021)

Detection of novel mutations that cause autosomal dominant retinitis pigmentosa in candidate genes by long-range PCR amplification and next-generation sequencing
por: Dias, Miguel de Sousa, et al.
Publicado: (2013)

Long-range PCR amplification-based targeted enrichment & next generation sequencing: A cost-effective testing strategy for lysosomal storage disorders
por: Vanaja, Maria Celestina, et al.
Publicado: (2023)

A method for counting PCR template molecules with application to next-generation sequencing
por: Casbon, James A., et al.
Publicado: (2011)

Dissecting Genomic Aberrations in Myeloproliferative Neoplasms by Multiplex-PCR and Next Generation Sequencing
por: Kirschner, Martin M. J., et al.
Publicado: (2015)

Multiplex PCR and Next Generation Sequencing for the Non-Invasive Detection of Bladder Cancer
por: Ward, Douglas G., et al.
Publicado: (2016)

Soybean Viromes in the Republic of Korea Revealed by RT-PCR and Next-Generation Sequencing
por: Jo, Yeonhwa, et al.
Publicado: (2020)

Integrative Analysis of Next-Generation Sequencing for Next-Generation Cancer Research toward Artificial Intelligence
por: Park, Youngjun, et al.
Publicado: (2021)

Sequence Depth, Not PCR Replication, Improves Ecological Inference from Next Generation DNA Sequencing
por: Smith, Dylan P., et al.
Publicado: (2014)

Multiplex profiling of serum proteins in solution using barcoded antibody fragments and next generation sequencing
por: Brofelth, Mattias, et al.
Publicado: (2020)

Comprehensive Analysis of Disease-Related Genes in Chronic Lymphocytic Leukemia by Multiplex PCR-Based Next Generation Sequencing
por: Vollbrecht, Claudia, et al.
Publicado: (2015)

Exploring benzimidazole resistance in Haemonchus contortus by next generation sequencing and droplet digital PCR
por: Baltrušis, Paulius, et al.
Publicado: (2018)

Next-generation sequencing and PCR technologies in monitoring the hospital microbiome and its drug resistance
por: Cason, Carolina, et al.
Publicado: (2022)

Comparison of Sequence Reads Obtained from Three Next-Generation Sequencing Platforms
por: Suzuki, Shingo, et al.
Publicado: (2011)

Novel bi-allelic variants expand the SPTBN4-related genetic and phenotypic spectrum
por: Buelow, Markus, et al.
Publicado: (2021)

Targeted next generation sequencing as a tool for precision medicine
por: Gulilat, Markus, et al.
Publicado: (2019)

Next-Generation Sequencing
por: Xiong, Momiao, et al.
Publicado: (2010)

Next-generation sequencing
por: Reis-Filho, Jorge S
Publicado: (2009)

Revealing the Mysteries of Acute Myeloid Leukemia: From Quantitative PCR through Next-Generation Sequencing and Systemic Metabolomic Profiling
por: Panuzzo, Cristina, et al.
Publicado: (2022)

Next generation sequencing technologies for next generation plant breeding
por: Ray, Soham, et al.
Publicado: (2014)

NGSNGS: next-generation simulator for next-generation sequencing data
por: Henriksen, Rasmus Amund, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_3nvqnpvtjch24ldrjvu9351mfd