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Selection of reliable reference genes for quantitative real-time PCR in human T cells and neutrophils

BACKGROUND: The choice of reliable reference genes is a prerequisite for valid results when analyzing gene expression with real-time quantitative PCR (qPCR). This method is frequently applied to study gene expression patterns in immune cells, yet a thorough validation of potential reference genes is...

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Detalles Bibliográficos
Autores principales: Ledderose, Carola, Heyn, Jens, Limbeck, Elisabeth, Kreth, Simone
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229292/
https://www.ncbi.nlm.nih.gov/pubmed/22011438
http://dx.doi.org/10.1186/1756-0500-4-427