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Neighborhood Properties Are Important Determinants of Temperature Sensitive Mutations
Temperature-sensitive (TS) mutants are powerful tools to study gene function in vivo. These mutants exhibit wild-type activity at permissive temperatures and reduced activity at restrictive temperatures. Although random mutagenesis can be used to generate TS mutants, the procedure is laborious and u...
Autores principales: | Lockwood, Svetlana, Krishnamoorthy, Bala, Ye, Ping |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3229608/ https://www.ncbi.nlm.nih.gov/pubmed/22164302 http://dx.doi.org/10.1371/journal.pone.0028507 |
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