The evaluation of yeast derivatives as adjuvants for the immune response to the Bm86 antigen in cattle

BACKGROUND: The Gavac™ vaccine against the cattle tick Boophilus microplus has proven its efficacy in a large number of controlled and field experiments. However, this vaccine could be further improved by searching for new alternative adjuvants that would induce a stronger long-lasting immune respon...

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Autores principales: Rodríguez Valle, Manuel, Montero, Carlos, Machado, Héctor, Joglar, Marisdania
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC32298/
https://www.ncbi.nlm.nih.gov/pubmed/11399206
http://dx.doi.org/10.1186/1472-6750-1-2
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author Rodríguez Valle, Manuel
Montero, Carlos
Machado, Héctor
Joglar, Marisdania
author_facet Rodríguez Valle, Manuel
Montero, Carlos
Machado, Héctor
Joglar, Marisdania
author_sort Rodríguez Valle, Manuel
collection PubMed
description BACKGROUND: The Gavac™ vaccine against the cattle tick Boophilus microplus has proven its efficacy in a large number of controlled and field experiments. However, this vaccine could be further improved by searching for new alternative adjuvants that would induce a stronger long-lasting immune response. We conducted several experiments to assay the adjuvant effect of fractions of the recombinant yeast Pichia pastoris in mouse and cattle models. In previous experiments, the combination of the yeast membrane with saponin was the most effective formulation in stimulating the humoral immune response in mice, eliciting a response higher than Montanide 888. The response was predominantly of the IgG1 isotype. Here, we evaluated the response in cattle and compared the results with that obtained in mice. RESULTS: Bm86 on the membrane of P. pastoris plus saponin produced high antibody titers in cattle, though the protection level against tick infestations was lower when compared to Gavac™, probably due to a decrease in the IgG1/IgG2 ratio. The predictive value of the mouse model was studied through correlation analysis between the isotype levels in mice and the efficacy of formulations in cattle. Good correlation was established between the level of antibodies in mice and cattle, and between the amount of anti -Bm86 IgG1 in mice and the degree of protection in cattle. CONCLUSION: Mouse model have the potential to predict immunogenicity and efficacy of formulations in cattle. These results also support the use of the yeast expression system for recombinant vaccine formulations, enabling the prediction of more cost - effective formulations.
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spelling pubmed-322982001-06-13 The evaluation of yeast derivatives as adjuvants for the immune response to the Bm86 antigen in cattle Rodríguez Valle, Manuel Montero, Carlos Machado, Héctor Joglar, Marisdania BMC Biotechnol Research Article BACKGROUND: The Gavac™ vaccine against the cattle tick Boophilus microplus has proven its efficacy in a large number of controlled and field experiments. However, this vaccine could be further improved by searching for new alternative adjuvants that would induce a stronger long-lasting immune response. We conducted several experiments to assay the adjuvant effect of fractions of the recombinant yeast Pichia pastoris in mouse and cattle models. In previous experiments, the combination of the yeast membrane with saponin was the most effective formulation in stimulating the humoral immune response in mice, eliciting a response higher than Montanide 888. The response was predominantly of the IgG1 isotype. Here, we evaluated the response in cattle and compared the results with that obtained in mice. RESULTS: Bm86 on the membrane of P. pastoris plus saponin produced high antibody titers in cattle, though the protection level against tick infestations was lower when compared to Gavac™, probably due to a decrease in the IgG1/IgG2 ratio. The predictive value of the mouse model was studied through correlation analysis between the isotype levels in mice and the efficacy of formulations in cattle. Good correlation was established between the level of antibodies in mice and cattle, and between the amount of anti -Bm86 IgG1 in mice and the degree of protection in cattle. CONCLUSION: Mouse model have the potential to predict immunogenicity and efficacy of formulations in cattle. These results also support the use of the yeast expression system for recombinant vaccine formulations, enabling the prediction of more cost - effective formulations. BioMed Central 2001-05-24 /pmc/articles/PMC32298/ /pubmed/11399206 http://dx.doi.org/10.1186/1472-6750-1-2 Text en Copyright © 2001 Rodríguez Valle et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Rodríguez Valle, Manuel
Montero, Carlos
Machado, Héctor
Joglar, Marisdania
The evaluation of yeast derivatives as adjuvants for the immune response to the Bm86 antigen in cattle
title The evaluation of yeast derivatives as adjuvants for the immune response to the Bm86 antigen in cattle
title_full The evaluation of yeast derivatives as adjuvants for the immune response to the Bm86 antigen in cattle
title_fullStr The evaluation of yeast derivatives as adjuvants for the immune response to the Bm86 antigen in cattle
title_full_unstemmed The evaluation of yeast derivatives as adjuvants for the immune response to the Bm86 antigen in cattle
title_short The evaluation of yeast derivatives as adjuvants for the immune response to the Bm86 antigen in cattle
title_sort evaluation of yeast derivatives as adjuvants for the immune response to the bm86 antigen in cattle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC32298/
https://www.ncbi.nlm.nih.gov/pubmed/11399206
http://dx.doi.org/10.1186/1472-6750-1-2
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