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Pu-erh Tea Inhibits Tumor Cell Growth by Down-Regulating Mutant p53

Pu-erh tea is a kind of fermented tea with the incorporation of microorganisms’ metabolites. Unlike green tea, the chemical characteristics and bioactivities of Pu-erh tea are still not well understood. Using water extracts of Pu-erh tea, we analyzed the tumor cell growth inhibition activities on se...

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Detalles Bibliográficos
Autores principales: Zhao, Lanjun, Jia, Shuting, Tang, Wenru, Sheng, Jun, Luo, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233424/
https://www.ncbi.nlm.nih.gov/pubmed/22174618
http://dx.doi.org/10.3390/ijms12117581
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author Zhao, Lanjun
Jia, Shuting
Tang, Wenru
Sheng, Jun
Luo, Ying
author_facet Zhao, Lanjun
Jia, Shuting
Tang, Wenru
Sheng, Jun
Luo, Ying
author_sort Zhao, Lanjun
collection PubMed
description Pu-erh tea is a kind of fermented tea with the incorporation of microorganisms’ metabolites. Unlike green tea, the chemical characteristics and bioactivities of Pu-erh tea are still not well understood. Using water extracts of Pu-erh tea, we analyzed the tumor cell growth inhibition activities on several genetically engineered mouse tumor cell lines. We found that at the concentration that did not affect wild type mouse embryo fibroblasts (MEFs) growth, Pu-erh tea extracts could inhibit tumor cell growth by down-regulated S phase and cause G1 or G2 arrest. Further study showed that Pu-erh tea extracts down-regulated the expression of mutant p53 in tumor cells at the protein level as well as mRNA level. The same concentration of Pu-erh tea solution did not cause p53 stabilization or activation of its downstream pathways in wild type cells. We also found that Pu-erh tea treatment could slightly down-regulate both HSP70 and HSP90 protein levels in tumor cells. These data revealed the action of Pu-erh tea on tumor cells and provided the possible mechanism for Pu-erh tea action, which explained its selectivity in inhibiting tumor cells without affecting wild type cells. Our data sheds light on the application of Pu-erh tea as an anti-tumor agent with low side effects.
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spelling pubmed-32334242011-12-15 Pu-erh Tea Inhibits Tumor Cell Growth by Down-Regulating Mutant p53 Zhao, Lanjun Jia, Shuting Tang, Wenru Sheng, Jun Luo, Ying Int J Mol Sci Article Pu-erh tea is a kind of fermented tea with the incorporation of microorganisms’ metabolites. Unlike green tea, the chemical characteristics and bioactivities of Pu-erh tea are still not well understood. Using water extracts of Pu-erh tea, we analyzed the tumor cell growth inhibition activities on several genetically engineered mouse tumor cell lines. We found that at the concentration that did not affect wild type mouse embryo fibroblasts (MEFs) growth, Pu-erh tea extracts could inhibit tumor cell growth by down-regulated S phase and cause G1 or G2 arrest. Further study showed that Pu-erh tea extracts down-regulated the expression of mutant p53 in tumor cells at the protein level as well as mRNA level. The same concentration of Pu-erh tea solution did not cause p53 stabilization or activation of its downstream pathways in wild type cells. We also found that Pu-erh tea treatment could slightly down-regulate both HSP70 and HSP90 protein levels in tumor cells. These data revealed the action of Pu-erh tea on tumor cells and provided the possible mechanism for Pu-erh tea action, which explained its selectivity in inhibiting tumor cells without affecting wild type cells. Our data sheds light on the application of Pu-erh tea as an anti-tumor agent with low side effects. Molecular Diversity Preservation International (MDPI) 2011-11-07 /pmc/articles/PMC3233424/ /pubmed/22174618 http://dx.doi.org/10.3390/ijms12117581 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhao, Lanjun
Jia, Shuting
Tang, Wenru
Sheng, Jun
Luo, Ying
Pu-erh Tea Inhibits Tumor Cell Growth by Down-Regulating Mutant p53
title Pu-erh Tea Inhibits Tumor Cell Growth by Down-Regulating Mutant p53
title_full Pu-erh Tea Inhibits Tumor Cell Growth by Down-Regulating Mutant p53
title_fullStr Pu-erh Tea Inhibits Tumor Cell Growth by Down-Regulating Mutant p53
title_full_unstemmed Pu-erh Tea Inhibits Tumor Cell Growth by Down-Regulating Mutant p53
title_short Pu-erh Tea Inhibits Tumor Cell Growth by Down-Regulating Mutant p53
title_sort pu-erh tea inhibits tumor cell growth by down-regulating mutant p53
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3233424/
https://www.ncbi.nlm.nih.gov/pubmed/22174618
http://dx.doi.org/10.3390/ijms12117581
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