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Cell-Free Protein Expression under Macromolecular Crowding Conditions

BACKGROUND: Cell-free protein expression (CFPE) comprised of in vitro transcription and translation is currently manipulated in relatively dilute solutions, in which the macromolecular crowding effects present in living cells are largely ignored. This may not only affect the efficiency of protein sy...

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Detalles Bibliográficos
Autores principales: Ge, Xumeng, Luo, Dan, Xu, Jianfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3234285/
https://www.ncbi.nlm.nih.gov/pubmed/22174874
http://dx.doi.org/10.1371/journal.pone.0028707