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Cell-Free Protein Expression under Macromolecular Crowding Conditions
BACKGROUND: Cell-free protein expression (CFPE) comprised of in vitro transcription and translation is currently manipulated in relatively dilute solutions, in which the macromolecular crowding effects present in living cells are largely ignored. This may not only affect the efficiency of protein sy...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2011
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3234285/ https://www.ncbi.nlm.nih.gov/pubmed/22174874 http://dx.doi.org/10.1371/journal.pone.0028707 |