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Isolation and characterization of human spermatogonial stem cells

BACKGROUND: To isolate and characterization of human spermatogonial stem cells from stem spermatogonium. METHODS: The disassociation of spermatogonial stem cells (SSCs) were performed using enzymatic digestion of type I collagenase and trypsin. The SSCs were isolated by using Percoll density gradien...

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Detalles Bibliográficos
Autores principales: Liu, Shixue, Tang, Ziwei, Xiong, Tao, Tang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3235066/
https://www.ncbi.nlm.nih.gov/pubmed/22018465
http://dx.doi.org/10.1186/1477-7827-9-141