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Heterogeneous Expression of Drosophila Gustatory Receptors in Enteroendocrine Cells

The gastrointestinal tract is emerging as a major site of chemosensation in mammalian studies. Enteroendocrine cells are chemosensory cells in the gut which produce regulatory peptides in response to luminal contents to regulate gut physiology, food intake, and glucose homeostasis, among other possi...

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Autores principales: Park, Jeong-Ho, Kwon, Jae Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237578/
https://www.ncbi.nlm.nih.gov/pubmed/22194978
http://dx.doi.org/10.1371/journal.pone.0029022
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author Park, Jeong-Ho
Kwon, Jae Young
author_facet Park, Jeong-Ho
Kwon, Jae Young
author_sort Park, Jeong-Ho
collection PubMed
description The gastrointestinal tract is emerging as a major site of chemosensation in mammalian studies. Enteroendocrine cells are chemosensory cells in the gut which produce regulatory peptides in response to luminal contents to regulate gut physiology, food intake, and glucose homeostasis, among other possible functions. Increasing evidence shows that mammalian taste receptors and taste signaling molecules are expressed in enteroendocrine cells in the gut. Invertebrate models such as Drosophila can provide a simple and genetically tractable system to study the chemosensory functions of enteroendocrine cells in vivo. To establish Drosophila enteroendocrine cells as a model for studying gut chemosensation, we used the GAL4/UAS system to examine the expression of all 68 Gustatory receptors (Grs) in the intestine. We find that 12 Gr-GAL4 drivers label subsets of enteroendocrine cells in the midgut, and examine colocalization of these drivers with the regulatory peptides neuropeptide F (NPF), locustatachykinin (LTK), and diuretic hormone 31 (DH31). RT-PCR analysis provides additional evidence for the presence of Gr transcripts in the gut. Our results suggest that the Drosophila Grs have chemosensory roles in the intestine to regulate physiological functions such as food uptake, nutrient absorption, or sugar homeostasis.
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spelling pubmed-32375782011-12-22 Heterogeneous Expression of Drosophila Gustatory Receptors in Enteroendocrine Cells Park, Jeong-Ho Kwon, Jae Young PLoS One Research Article The gastrointestinal tract is emerging as a major site of chemosensation in mammalian studies. Enteroendocrine cells are chemosensory cells in the gut which produce regulatory peptides in response to luminal contents to regulate gut physiology, food intake, and glucose homeostasis, among other possible functions. Increasing evidence shows that mammalian taste receptors and taste signaling molecules are expressed in enteroendocrine cells in the gut. Invertebrate models such as Drosophila can provide a simple and genetically tractable system to study the chemosensory functions of enteroendocrine cells in vivo. To establish Drosophila enteroendocrine cells as a model for studying gut chemosensation, we used the GAL4/UAS system to examine the expression of all 68 Gustatory receptors (Grs) in the intestine. We find that 12 Gr-GAL4 drivers label subsets of enteroendocrine cells in the midgut, and examine colocalization of these drivers with the regulatory peptides neuropeptide F (NPF), locustatachykinin (LTK), and diuretic hormone 31 (DH31). RT-PCR analysis provides additional evidence for the presence of Gr transcripts in the gut. Our results suggest that the Drosophila Grs have chemosensory roles in the intestine to regulate physiological functions such as food uptake, nutrient absorption, or sugar homeostasis. Public Library of Science 2011-12-14 /pmc/articles/PMC3237578/ /pubmed/22194978 http://dx.doi.org/10.1371/journal.pone.0029022 Text en Park, Kwon. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Park, Jeong-Ho
Kwon, Jae Young
Heterogeneous Expression of Drosophila Gustatory Receptors in Enteroendocrine Cells
title Heterogeneous Expression of Drosophila Gustatory Receptors in Enteroendocrine Cells
title_full Heterogeneous Expression of Drosophila Gustatory Receptors in Enteroendocrine Cells
title_fullStr Heterogeneous Expression of Drosophila Gustatory Receptors in Enteroendocrine Cells
title_full_unstemmed Heterogeneous Expression of Drosophila Gustatory Receptors in Enteroendocrine Cells
title_short Heterogeneous Expression of Drosophila Gustatory Receptors in Enteroendocrine Cells
title_sort heterogeneous expression of drosophila gustatory receptors in enteroendocrine cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237578/
https://www.ncbi.nlm.nih.gov/pubmed/22194978
http://dx.doi.org/10.1371/journal.pone.0029022
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