Establishment of Methylation-Specific PCR for the Mouse p53 Gene

Methylation-specific PCR (MSP) of the mouse p53 gene has not yet been reported. We searched the CpG islands, sequenced the bisulfited DNA, and designed PCR primers for methylation and unmethylation sites. DNA from a young mouse produced a strong PCR product with the unmethylated primer and a weaker...

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Detalles Bibliográficos
Autores principales: Okazaki, Ryuji, Ootsuyama, Akira, Yoshida, Yasuhiro, Norimura, Toshiyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3238407/
https://www.ncbi.nlm.nih.gov/pubmed/22203902
http://dx.doi.org/10.4061/2011/938435

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3238407/
https://www.ncbi.nlm.nih.gov/pubmed/22203902
http://dx.doi.org/10.4061/2011/938435

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