Direct cloning of double-stranded RNAs from RNase protection analysis reveals processing patterns of C/D box snoRNAs and provides evidence for widespread antisense transcript expression

We describe a new method that allows cloning of double-stranded RNAs (dsRNAs) that are generated in RNase protection experiments. We demonstrate that the mouse C/D box snoRNA MBII-85 (SNORD116) is processed into at least five shorter RNAs using processing sites near known functional elements of C/D...

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Detalles Bibliográficos
Autores principales: Shen, Manli, Eyras, Eduardo, Wu, Jie, Khanna, Amit, Josiah, Serene, Rederstorff, Mathieu, Zhang, Michael Q., Stamm, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3239178/
https://www.ncbi.nlm.nih.gov/pubmed/21880592
http://dx.doi.org/10.1093/nar/gkr684