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Stacking of G-quadruplexes: NMR structure of a G-rich oligonucleotide with potential anti-HIV and anticancer activity(†)

G-rich oligonucleotides T30695 (or T30923), with the sequence of (GGGT)(4), and T40214, with the sequence of (GGGC)(4), have been reported to exhibit anti-HIV and anticancer activity. Here we report on the structure of a dimeric G-quadruplex adopted by a derivative of these sequences in K(+) solutio...

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Autores principales: Do, Ngoc Quang, Lim, Kah Wai, Teo, Ming Hoon, Heddi, Brahim, Phan, Anh Tuân
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241632/
https://www.ncbi.nlm.nih.gov/pubmed/21840903
http://dx.doi.org/10.1093/nar/gkr539
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author Do, Ngoc Quang
Lim, Kah Wai
Teo, Ming Hoon
Heddi, Brahim
Phan, Anh Tuân
author_facet Do, Ngoc Quang
Lim, Kah Wai
Teo, Ming Hoon
Heddi, Brahim
Phan, Anh Tuân
author_sort Do, Ngoc Quang
collection PubMed
description G-rich oligonucleotides T30695 (or T30923), with the sequence of (GGGT)(4), and T40214, with the sequence of (GGGC)(4), have been reported to exhibit anti-HIV and anticancer activity. Here we report on the structure of a dimeric G-quadruplex adopted by a derivative of these sequences in K(+) solution. It comprises two identical propeller-type parallel-stranded G-quadruplex subunits each containing three G-tetrad layers that are stacked via the 5′-5′ interface. We demonstrated control over the stacking of the two monomeric subunits by sequence modifications. Our analysis of possible structures at the stacking interface provides a general principle for stacking of G-quadruplexes, which could have implications for the assembly and recognition of higher-order G-quadruplex structures.
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spelling pubmed-32416322011-12-19 Stacking of G-quadruplexes: NMR structure of a G-rich oligonucleotide with potential anti-HIV and anticancer activity(†) Do, Ngoc Quang Lim, Kah Wai Teo, Ming Hoon Heddi, Brahim Phan, Anh Tuân Nucleic Acids Res Structural Biology G-rich oligonucleotides T30695 (or T30923), with the sequence of (GGGT)(4), and T40214, with the sequence of (GGGC)(4), have been reported to exhibit anti-HIV and anticancer activity. Here we report on the structure of a dimeric G-quadruplex adopted by a derivative of these sequences in K(+) solution. It comprises two identical propeller-type parallel-stranded G-quadruplex subunits each containing three G-tetrad layers that are stacked via the 5′-5′ interface. We demonstrated control over the stacking of the two monomeric subunits by sequence modifications. Our analysis of possible structures at the stacking interface provides a general principle for stacking of G-quadruplexes, which could have implications for the assembly and recognition of higher-order G-quadruplex structures. Oxford University Press 2011-11 2011-08-12 /pmc/articles/PMC3241632/ /pubmed/21840903 http://dx.doi.org/10.1093/nar/gkr539 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Structural Biology
Do, Ngoc Quang
Lim, Kah Wai
Teo, Ming Hoon
Heddi, Brahim
Phan, Anh Tuân
Stacking of G-quadruplexes: NMR structure of a G-rich oligonucleotide with potential anti-HIV and anticancer activity(†)
title Stacking of G-quadruplexes: NMR structure of a G-rich oligonucleotide with potential anti-HIV and anticancer activity(†)
title_full Stacking of G-quadruplexes: NMR structure of a G-rich oligonucleotide with potential anti-HIV and anticancer activity(†)
title_fullStr Stacking of G-quadruplexes: NMR structure of a G-rich oligonucleotide with potential anti-HIV and anticancer activity(†)
title_full_unstemmed Stacking of G-quadruplexes: NMR structure of a G-rich oligonucleotide with potential anti-HIV and anticancer activity(†)
title_short Stacking of G-quadruplexes: NMR structure of a G-rich oligonucleotide with potential anti-HIV and anticancer activity(†)
title_sort stacking of g-quadruplexes: nmr structure of a g-rich oligonucleotide with potential anti-hiv and anticancer activity(†)
topic Structural Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3241632/
https://www.ncbi.nlm.nih.gov/pubmed/21840903
http://dx.doi.org/10.1093/nar/gkr539
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