A Cell-Based Protein-Protein Interaction Method Using a Permuted Luciferase Reporter

We have developed a novel cell-based protein-protein interaction assay method. The method relies on conversion of an inactive permuted luciferase containing a Tobacco Etch Virus protease (TEV) cleavage sequence fused onto protein (A) to an active luciferase upon interaction and cleavage by another p...

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Detalles Bibliográficos
Autores principales: Eishingdrelo, Haifeng, Cai, Jidong, Weissensee, Paul, Sharma, Praveen, Tocci, Michael J, Wright, Paul S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242404/
https://www.ncbi.nlm.nih.gov/pubmed/22207892
http://dx.doi.org/10.2174/1875397301105010122

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242404/
https://www.ncbi.nlm.nih.gov/pubmed/22207892
http://dx.doi.org/10.2174/1875397301105010122

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