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A Cell-Based Protein-Protein Interaction Method Using a Permuted Luciferase Reporter
We have developed a novel cell-based protein-protein interaction assay method. The method relies on conversion of an inactive permuted luciferase containing a Tobacco Etch Virus protease (TEV) cleavage sequence fused onto protein (A) to an active luciferase upon interaction and cleavage by another p...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Bentham Open
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242404/ https://www.ncbi.nlm.nih.gov/pubmed/22207892 http://dx.doi.org/10.2174/1875397301105010122 |