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PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis
The microtubule-organizing activity of the centrosome oscillates during the cell cycle, reaching its highest level at mitosis. At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar matrix (PCM) and a robust increase in micro...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246884/ https://www.ncbi.nlm.nih.gov/pubmed/22184200 http://dx.doi.org/10.1083/jcb.201106093 |
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author | Lee, Kwanwoo Rhee, Kunsoo |
author_facet | Lee, Kwanwoo Rhee, Kunsoo |
author_sort | Lee, Kwanwoo |
collection | PubMed |
description | The microtubule-organizing activity of the centrosome oscillates during the cell cycle, reaching its highest level at mitosis. At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar matrix (PCM) and a robust increase in microtubule-organizing activity. It is known that PLK1 is critical for the initiation of centrosome maturation. In this paper, we report that pericentrin (PCNT), a PCM protein, was specifically phosphorylated by PLK1 during mitosis. Phosphoresistant point mutants of PCNT did not recruit centrosomal proteins, such as CEP192, GCP-WD (γ-complex protein with WD repeats), γ-tubulin, Aurora A, and PLK1, into the centrosome during mitosis. However, centrosomal recruitment of CEP215 depended on PCNT irrespective of its phosphorylation status. Furthermore, ectopic expression of PLK1-PCNT fusion proteins induced the centrosomal accumulation of CEP192, GCP-WD, and γ-tubulin even in interphase cells, mimicking centrosome maturation. Based on these results, we propose that PLK1-mediated phosphorylation of PCNT initiates centrosome maturation by organizing the spindle pole–specific PCM lattice. |
format | Online Article Text |
id | pubmed-3246884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-32468842012-06-26 PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis Lee, Kwanwoo Rhee, Kunsoo J Cell Biol Research Articles The microtubule-organizing activity of the centrosome oscillates during the cell cycle, reaching its highest level at mitosis. At the onset of mitosis, the centrosome undergoes maturation, which is characterized by a drastic expansion of the pericentriolar matrix (PCM) and a robust increase in microtubule-organizing activity. It is known that PLK1 is critical for the initiation of centrosome maturation. In this paper, we report that pericentrin (PCNT), a PCM protein, was specifically phosphorylated by PLK1 during mitosis. Phosphoresistant point mutants of PCNT did not recruit centrosomal proteins, such as CEP192, GCP-WD (γ-complex protein with WD repeats), γ-tubulin, Aurora A, and PLK1, into the centrosome during mitosis. However, centrosomal recruitment of CEP215 depended on PCNT irrespective of its phosphorylation status. Furthermore, ectopic expression of PLK1-PCNT fusion proteins induced the centrosomal accumulation of CEP192, GCP-WD, and γ-tubulin even in interphase cells, mimicking centrosome maturation. Based on these results, we propose that PLK1-mediated phosphorylation of PCNT initiates centrosome maturation by organizing the spindle pole–specific PCM lattice. The Rockefeller University Press 2011-12-26 /pmc/articles/PMC3246884/ /pubmed/22184200 http://dx.doi.org/10.1083/jcb.201106093 Text en © 2011 Lee and Rhee This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Lee, Kwanwoo Rhee, Kunsoo PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis |
title | PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis |
title_full | PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis |
title_fullStr | PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis |
title_full_unstemmed | PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis |
title_short | PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis |
title_sort | plk1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246884/ https://www.ncbi.nlm.nih.gov/pubmed/22184200 http://dx.doi.org/10.1083/jcb.201106093 |
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