Ascorbate and thiol antioxidants abolish sensitivity of yeast Saccharomyces cerevisiae to disulfiram

Sensitivity of baker’s yeast to disulfiram (DSF) and hypersensitivity of a mutant devoid of Cu, Zn-superoxide dismutase to this compound is reported, demonstrating that yeast may be a simple convenient eukaryotic model to study the mechanism of DSF toxicity. DSF was found to induce oxidative stress...

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Autores principales: Kwolek-Mirek, Magdalena, Zadrag-Tecza, Renata, Bartosz, Grzegorz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2011
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247666/
https://www.ncbi.nlm.nih.gov/pubmed/21866320
http://dx.doi.org/10.1007/s10565-011-9200-z
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author Kwolek-Mirek, Magdalena
Zadrag-Tecza, Renata
Bartosz, Grzegorz
author_facet Kwolek-Mirek, Magdalena
Zadrag-Tecza, Renata
Bartosz, Grzegorz
author_sort Kwolek-Mirek, Magdalena
collection PubMed
description Sensitivity of baker’s yeast to disulfiram (DSF) and hypersensitivity of a mutant devoid of Cu, Zn-superoxide dismutase to this compound is reported, demonstrating that yeast may be a simple convenient eukaryotic model to study the mechanism of DSF toxicity. DSF was found to induce oxidative stress in yeast cells demonstrated by increased superoxide production and decrease of cellular glutathione content. Anoxic atmosphere and hydrophilic antioxidants (ascorbate, glutathione, dithiothreitol, cysteine, and N-acetylcysteine) ameliorated DSF toxicity to yeast indicating that oxidative stress plays a critical role in the cellular action of DSF.
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spelling pubmed-32476662011-12-29 Ascorbate and thiol antioxidants abolish sensitivity of yeast Saccharomyces cerevisiae to disulfiram Kwolek-Mirek, Magdalena Zadrag-Tecza, Renata Bartosz, Grzegorz Cell Biol Toxicol Original Research Sensitivity of baker’s yeast to disulfiram (DSF) and hypersensitivity of a mutant devoid of Cu, Zn-superoxide dismutase to this compound is reported, demonstrating that yeast may be a simple convenient eukaryotic model to study the mechanism of DSF toxicity. DSF was found to induce oxidative stress in yeast cells demonstrated by increased superoxide production and decrease of cellular glutathione content. Anoxic atmosphere and hydrophilic antioxidants (ascorbate, glutathione, dithiothreitol, cysteine, and N-acetylcysteine) ameliorated DSF toxicity to yeast indicating that oxidative stress plays a critical role in the cellular action of DSF. Springer Netherlands 2011-08-25 2012 /pmc/articles/PMC3247666/ /pubmed/21866320 http://dx.doi.org/10.1007/s10565-011-9200-z Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Research
Kwolek-Mirek, Magdalena
Zadrag-Tecza, Renata
Bartosz, Grzegorz
Ascorbate and thiol antioxidants abolish sensitivity of yeast Saccharomyces cerevisiae to disulfiram
title Ascorbate and thiol antioxidants abolish sensitivity of yeast Saccharomyces cerevisiae to disulfiram
title_full Ascorbate and thiol antioxidants abolish sensitivity of yeast Saccharomyces cerevisiae to disulfiram
title_fullStr Ascorbate and thiol antioxidants abolish sensitivity of yeast Saccharomyces cerevisiae to disulfiram
title_full_unstemmed Ascorbate and thiol antioxidants abolish sensitivity of yeast Saccharomyces cerevisiae to disulfiram
title_short Ascorbate and thiol antioxidants abolish sensitivity of yeast Saccharomyces cerevisiae to disulfiram
title_sort ascorbate and thiol antioxidants abolish sensitivity of yeast saccharomyces cerevisiae to disulfiram
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247666/
https://www.ncbi.nlm.nih.gov/pubmed/21866320
http://dx.doi.org/10.1007/s10565-011-9200-z
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