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RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor

The evolutionarily conserved Pat1 proteins are P-body components recently shown to play important roles in cytoplasmic gene expression control. Using human cell lines, we demonstrate that human Pat1b is a shuttling protein whose nuclear export is mediated via a consensus NES sequence and Crm1, as ev...

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Autores principales: Marnef, Aline, Weil, Dominique, Standart, Nancy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248899/
https://www.ncbi.nlm.nih.gov/pubmed/22090346
http://dx.doi.org/10.1091/mbc.E11-05-0415
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author Marnef, Aline
Weil, Dominique
Standart, Nancy
author_facet Marnef, Aline
Weil, Dominique
Standart, Nancy
author_sort Marnef, Aline
collection PubMed
description The evolutionarily conserved Pat1 proteins are P-body components recently shown to play important roles in cytoplasmic gene expression control. Using human cell lines, we demonstrate that human Pat1b is a shuttling protein whose nuclear export is mediated via a consensus NES sequence and Crm1, as evidenced by leptomycin B (LMB) treatment. However, not all P-body components are nucleocytoplasmic proteins; rck/p54, Dcp1a, Edc3, Ge-1, and Xrn1 are insensitive to LMB and remain cytoplasmic in its presence. Nuclear Pat1b localizes to PML–associated foci and SC35-containing splicing speckles in a transcription-dependent manner, whereas in the absence of RNA synthesis, Pat1b redistributes to crescent-shaped nucleolar caps. Furthermore, inhibition of splicing by spliceostatin A leads to the reorganization of SC35 speckles, which is closely mirrored by Pat1b, indicating that it may also be involved in splicing processes. Of interest, Pat1b retention in these three nuclear compartments is mediated via distinct regions of the protein. Examination of the nuclear distribution of 4E-T(ransporter), an additional P-body nucleocytoplasmic protein, revealed that 4E-T colocalizes with Pat1b in PML-associated foci but not in nucleolar caps. Taken together, our findings strongly suggest that Pat1b participates in several RNA-related nuclear processes in addition to its multiple regulatory roles in the cytoplasm.
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spelling pubmed-32488992012-03-16 RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor Marnef, Aline Weil, Dominique Standart, Nancy Mol Biol Cell Articles The evolutionarily conserved Pat1 proteins are P-body components recently shown to play important roles in cytoplasmic gene expression control. Using human cell lines, we demonstrate that human Pat1b is a shuttling protein whose nuclear export is mediated via a consensus NES sequence and Crm1, as evidenced by leptomycin B (LMB) treatment. However, not all P-body components are nucleocytoplasmic proteins; rck/p54, Dcp1a, Edc3, Ge-1, and Xrn1 are insensitive to LMB and remain cytoplasmic in its presence. Nuclear Pat1b localizes to PML–associated foci and SC35-containing splicing speckles in a transcription-dependent manner, whereas in the absence of RNA synthesis, Pat1b redistributes to crescent-shaped nucleolar caps. Furthermore, inhibition of splicing by spliceostatin A leads to the reorganization of SC35 speckles, which is closely mirrored by Pat1b, indicating that it may also be involved in splicing processes. Of interest, Pat1b retention in these three nuclear compartments is mediated via distinct regions of the protein. Examination of the nuclear distribution of 4E-T(ransporter), an additional P-body nucleocytoplasmic protein, revealed that 4E-T colocalizes with Pat1b in PML-associated foci but not in nucleolar caps. Taken together, our findings strongly suggest that Pat1b participates in several RNA-related nuclear processes in addition to its multiple regulatory roles in the cytoplasm. The American Society for Cell Biology 2012-01-01 /pmc/articles/PMC3248899/ /pubmed/22090346 http://dx.doi.org/10.1091/mbc.E11-05-0415 Text en © 2012 Marnef et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Marnef, Aline
Weil, Dominique
Standart, Nancy
RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor
title RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor
title_full RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor
title_fullStr RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor
title_full_unstemmed RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor
title_short RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor
title_sort rna-related nuclear functions of human pat1b, the p-body mrna decay factor
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248899/
https://www.ncbi.nlm.nih.gov/pubmed/22090346
http://dx.doi.org/10.1091/mbc.E11-05-0415
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