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The kinase activity of human Rio1 is required for final steps of cytoplasmic maturation of 40S subunits

RIO proteins form a conserved family of atypical protein kinases. Humans possess three distinct RIO kinases—hRio1, hRio2, and hRio3, of which only hRio2 has been characterized with respect to its role in ribosomal biogenesis. Here we show that both hRio1 and hRio3, like hRio2, are associated with pr...

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Autores principales: Widmann, Barbara, Wandrey, Franziska, Badertscher, Lukas, Wyler, Emanuel, Pfannstiel, Jens, Zemp, Ivo, Kutay, Ulrike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248900/
https://www.ncbi.nlm.nih.gov/pubmed/22072790
http://dx.doi.org/10.1091/mbc.E11-07-0639
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author Widmann, Barbara
Wandrey, Franziska
Badertscher, Lukas
Wyler, Emanuel
Pfannstiel, Jens
Zemp, Ivo
Kutay, Ulrike
author_facet Widmann, Barbara
Wandrey, Franziska
Badertscher, Lukas
Wyler, Emanuel
Pfannstiel, Jens
Zemp, Ivo
Kutay, Ulrike
author_sort Widmann, Barbara
collection PubMed
description RIO proteins form a conserved family of atypical protein kinases. Humans possess three distinct RIO kinases—hRio1, hRio2, and hRio3, of which only hRio2 has been characterized with respect to its role in ribosomal biogenesis. Here we show that both hRio1 and hRio3, like hRio2, are associated with precursors of 40S ribosomal subunits in human cells. Furthermore, we demonstrate that depletion of hRio1 by RNA interference affects the last step of 18S rRNA maturation and causes defects in the recycling of several trans-acting factors (hEnp1, hRio2, hLtv1, hDim2/PNO1, and hNob1) from pre-40S subunits in the cytoplasm. Although the effects of hRio1 and hRio2 depletion are similar, we show that the two kinases are not fully interchangeable. Moreover, rescue experiments with a kinase-dead mutant of hRio1 revealed that the kinase activity of hRio1 is essential for the recycling of the endonuclease hNob1 and its binding partner hDim2 from cytoplasmic pre-40S. Kinase-dead hRio1 is trapped on pre-40S particles containing hDim2 and hNob1 but devoid of hEnp1, hLtv1, and hRio2. These data reveal a role of hRio1 in the final stages of cytoplasmic pre-40S maturation.
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spelling pubmed-32489002012-03-16 The kinase activity of human Rio1 is required for final steps of cytoplasmic maturation of 40S subunits Widmann, Barbara Wandrey, Franziska Badertscher, Lukas Wyler, Emanuel Pfannstiel, Jens Zemp, Ivo Kutay, Ulrike Mol Biol Cell Articles RIO proteins form a conserved family of atypical protein kinases. Humans possess three distinct RIO kinases—hRio1, hRio2, and hRio3, of which only hRio2 has been characterized with respect to its role in ribosomal biogenesis. Here we show that both hRio1 and hRio3, like hRio2, are associated with precursors of 40S ribosomal subunits in human cells. Furthermore, we demonstrate that depletion of hRio1 by RNA interference affects the last step of 18S rRNA maturation and causes defects in the recycling of several trans-acting factors (hEnp1, hRio2, hLtv1, hDim2/PNO1, and hNob1) from pre-40S subunits in the cytoplasm. Although the effects of hRio1 and hRio2 depletion are similar, we show that the two kinases are not fully interchangeable. Moreover, rescue experiments with a kinase-dead mutant of hRio1 revealed that the kinase activity of hRio1 is essential for the recycling of the endonuclease hNob1 and its binding partner hDim2 from cytoplasmic pre-40S. Kinase-dead hRio1 is trapped on pre-40S particles containing hDim2 and hNob1 but devoid of hEnp1, hLtv1, and hRio2. These data reveal a role of hRio1 in the final stages of cytoplasmic pre-40S maturation. The American Society for Cell Biology 2012-01-01 /pmc/articles/PMC3248900/ /pubmed/22072790 http://dx.doi.org/10.1091/mbc.E11-07-0639 Text en © 2012 Widmann et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Widmann, Barbara
Wandrey, Franziska
Badertscher, Lukas
Wyler, Emanuel
Pfannstiel, Jens
Zemp, Ivo
Kutay, Ulrike
The kinase activity of human Rio1 is required for final steps of cytoplasmic maturation of 40S subunits
title The kinase activity of human Rio1 is required for final steps of cytoplasmic maturation of 40S subunits
title_full The kinase activity of human Rio1 is required for final steps of cytoplasmic maturation of 40S subunits
title_fullStr The kinase activity of human Rio1 is required for final steps of cytoplasmic maturation of 40S subunits
title_full_unstemmed The kinase activity of human Rio1 is required for final steps of cytoplasmic maturation of 40S subunits
title_short The kinase activity of human Rio1 is required for final steps of cytoplasmic maturation of 40S subunits
title_sort kinase activity of human rio1 is required for final steps of cytoplasmic maturation of 40s subunits
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248900/
https://www.ncbi.nlm.nih.gov/pubmed/22072790
http://dx.doi.org/10.1091/mbc.E11-07-0639
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