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GAL1-SceI directed site-specific genomic (gsSSG) mutagenesis: a method for precisely targeting point mutations in S. cerevisiae

BACKGROUND: Precise targeted mutations are defined as targeted mutations that do not require the retention of other genetic changes, such as marker genes, near the mutation site. In the yeast, S. cerevisiae, there are several methods for introducing precise targeted mutations, all of which depend on...

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Detalles Bibliográficos
Autores principales: Piccirillo, Sarah, Wang, Hsiao-Lin, Fisher, Thomas J, Honigberg, Saul M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3251539/
https://www.ncbi.nlm.nih.gov/pubmed/22141399
http://dx.doi.org/10.1186/1472-6750-11-120