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Generation of Long Insert Pairs Using a Cre-LoxP Inverse PCR Approach

Large insert mate pair reads have a major impact on the overall success of de novo assembly and the discovery of inherited and acquired structural variants. The positional information of mate pair reads generally improves genome assembly by resolving repeat elements and/or ordering contigs. Currentl...

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Detalles Bibliográficos
Autores principales: Peng, Ze, Zhao, Zhiying, Nath, Nandita, Froula, Jeff L., Clum, Alicia, Zhang, Tao, Cheng, Jan-fang, Copeland, Alex C., Pennacchio, Len A., Chen, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3253782/
https://www.ncbi.nlm.nih.gov/pubmed/22253722
http://dx.doi.org/10.1371/journal.pone.0029437