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A predicted hairpin cluster correlates with barriers to PCR, sequencing and possibly BAC recombineering
Formation of higher-order structure of nucleic acids (hairpins or loops, for example) may impact not only gene regulation, but also molecular biology techniques and approaches critical for design and production of vectors needed for genetic engineering approaches. In the course of designing vectors...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group
2011
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255507/ https://www.ncbi.nlm.nih.gov/pubmed/22355623 http://dx.doi.org/10.1038/srep00106 |