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Site directed biotinylation of filamentous phage structural proteins

Filamentous bacteriophages have been used in numerous applications for the display of antibodies and random peptide libraries. Here we describe the introduction of a 13 amino acid sequence LASIFEAQKIEWR (designated BT, which is biotinylated in vivo by E. coli) into the N termini of four of the five...

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Detalles Bibliográficos
Autores principales: Smelyanski, Larisa, Gershoni, Jonathan M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3256129/
https://www.ncbi.nlm.nih.gov/pubmed/22044460
http://dx.doi.org/10.1186/1743-422X-8-495