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Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR
Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples, the possible presence of inhibitors forms a c...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258155/ https://www.ncbi.nlm.nih.gov/pubmed/22102586 http://dx.doi.org/10.1093/nar/gkr775 |