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Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR

Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples, the possible presence of inhibitors forms a c...

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Detalles Bibliográficos
Autores principales: Lievens, Antoon, Van Aelst, S., Van den Bulcke, M., Goetghebeur, E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258155/
https://www.ncbi.nlm.nih.gov/pubmed/22102586
http://dx.doi.org/10.1093/nar/gkr775