High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer

This report describes the combined use of an enzyme-based glucose release system (EnBase(®)) and high-aeration shake flask (Ultra Yield Flask™). The benefit of this combination is demonstrated by over 100-fold improvement in the active yield of recombinant alcohol dehydrogenase expressed in E. coli....

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Detalles Bibliográficos
Autores principales: Ukkonen, Kaisa, Vasala, Antti, Ojamo, Heikki, Neubauer, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258199/
https://www.ncbi.nlm.nih.gov/pubmed/22152005
http://dx.doi.org/10.1186/1475-2859-10-107

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258199/
https://www.ncbi.nlm.nih.gov/pubmed/22152005
http://dx.doi.org/10.1186/1475-2859-10-107

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