Highly Parallel and Short-Acting Amplification with Locus-Specific Primers to Detect Single Nucleotide Polymorphisms by the DigiTag2 Assay

The DigiTag2 assay enables analysis of a set of 96 SNPs using Kapa 2GFast HotStart DNA polymerase with a new protocol that has a total running time of about 7 hours, which is 6 hours shorter than the previous protocol. Quality parameters (conversion rate, call rate, reproducibility and concordance)...

Descripción completa

Detalles Bibliográficos
Autores principales: Nishida, Nao, Mawatari, Yoriko, Sageshima, Megumi, Tokunaga, Katsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258256/
https://www.ncbi.nlm.nih.gov/pubmed/22253840
http://dx.doi.org/10.1371/journal.pone.0029967

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3258256/
https://www.ncbi.nlm.nih.gov/pubmed/22253840
http://dx.doi.org/10.1371/journal.pone.0029967

Ejemplares similares