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Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification
For the detection of wheat yellow mosaic virus (WYMV), we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method. Using Primer Explorer software, four sets of primers were designed and RT-LAMP assay reaction conditions were optimized. The RT-LAMP was performed at...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260119/ https://www.ncbi.nlm.nih.gov/pubmed/22185375 http://dx.doi.org/10.1186/1743-422X-8-550 |