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A Novel Universal Primer-Multiplex-PCR Method with Sequencing Gel Electrophoresis Analysis

In this study, a novel universal primer-multiplex-PCR (UP-M-PCR) method adding a universal primer (UP) in the multiplex PCR reaction system was described. A universal adapter was designed in the 5′-end of each specific primer pairs which matched with the specific DNA sequences for each template and...

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Detalles Bibliográficos
Autores principales: Xu, Wentao, Zhai, Zhifang, Huang, Kunlun, Zhang, Nan, Yuan, Yanfang, Shang, Ying, Luo, Yunbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260127/
https://www.ncbi.nlm.nih.gov/pubmed/22272223
http://dx.doi.org/10.1371/journal.pone.0022900