Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method

BACKGROUND: Transgenic strains of Caenorhabditis elegans are typically generated by injecting DNA into the germline to form multi-copy extrachromosomal arrays. These transgenes are semi-stable and their expression is silenced in the germline. Mos1 transposon or microparticle bombardment methods have...

Descripción completa

Detalles Bibliográficos
Autores principales: Kage-Nakadai, Eriko, Kobuna, Hiroyuki, Funatsu, Osamu, Otori, Muneyoshi, Gengyo-Ando, Keiko, Yoshina, Sawako, Hori, Sayaka, Mitani, Shohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3262153/
https://www.ncbi.nlm.nih.gov/pubmed/22217006
http://dx.doi.org/10.1186/1472-6750-12-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3262153/
https://www.ncbi.nlm.nih.gov/pubmed/22217006
http://dx.doi.org/10.1186/1472-6750-12-1

Ejemplares similares