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Mice completely lacking immunoproteasomes display major alterations in antigen presentation
The importance of immunoproteasomes to antigen presentation has been unclear because animals totally lacking immunoproteasomes have not been previously developed. Here we show that dendritic cells from mice lacking the three immunoproteasome catalytic subunits display defects in presenting multiple...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3262888/ https://www.ncbi.nlm.nih.gov/pubmed/22197977 http://dx.doi.org/10.1038/ni.2203 |
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author | Kincaid, Eleanor Z Che, Jenny W York, Ian Escobar, Hernando Reyes-Vargas, Eduardo Delgado, Julio C. Welsh, Raymond M Karow, Margaret L. Murphy, Andrew J. Valenzuela, David M. Yancopoulos, George D. Rock, Kenneth L |
author_facet | Kincaid, Eleanor Z Che, Jenny W York, Ian Escobar, Hernando Reyes-Vargas, Eduardo Delgado, Julio C. Welsh, Raymond M Karow, Margaret L. Murphy, Andrew J. Valenzuela, David M. Yancopoulos, George D. Rock, Kenneth L |
author_sort | Kincaid, Eleanor Z |
collection | PubMed |
description | The importance of immunoproteasomes to antigen presentation has been unclear because animals totally lacking immunoproteasomes have not been previously developed. Here we show that dendritic cells from mice lacking the three immunoproteasome catalytic subunits display defects in presenting multiple major histocompatability (MHC) class I epitopes. During viral infection in vivo, the presentation of a majority of MHC class I epitopes is markedly reduced in immunoproteasome-deficient animals, while presentation of MHC class II peptides is unaffected. By mass spectrometry the repertoire of MHC class I-presented peptides is ~50% different and these differences are sufficient to stimulate robust transplant rejection of wild type cells in mutant mice. These results indicate that immunoproteasomes play a much more important role in antigen presentation than previously thought. |
format | Online Article Text |
id | pubmed-3262888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
record_format | MEDLINE/PubMed |
spelling | pubmed-32628882012-08-01 Mice completely lacking immunoproteasomes display major alterations in antigen presentation Kincaid, Eleanor Z Che, Jenny W York, Ian Escobar, Hernando Reyes-Vargas, Eduardo Delgado, Julio C. Welsh, Raymond M Karow, Margaret L. Murphy, Andrew J. Valenzuela, David M. Yancopoulos, George D. Rock, Kenneth L Nat Immunol Article The importance of immunoproteasomes to antigen presentation has been unclear because animals totally lacking immunoproteasomes have not been previously developed. Here we show that dendritic cells from mice lacking the three immunoproteasome catalytic subunits display defects in presenting multiple major histocompatability (MHC) class I epitopes. During viral infection in vivo, the presentation of a majority of MHC class I epitopes is markedly reduced in immunoproteasome-deficient animals, while presentation of MHC class II peptides is unaffected. By mass spectrometry the repertoire of MHC class I-presented peptides is ~50% different and these differences are sufficient to stimulate robust transplant rejection of wild type cells in mutant mice. These results indicate that immunoproteasomes play a much more important role in antigen presentation than previously thought. 2011-12-25 /pmc/articles/PMC3262888/ /pubmed/22197977 http://dx.doi.org/10.1038/ni.2203 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Kincaid, Eleanor Z Che, Jenny W York, Ian Escobar, Hernando Reyes-Vargas, Eduardo Delgado, Julio C. Welsh, Raymond M Karow, Margaret L. Murphy, Andrew J. Valenzuela, David M. Yancopoulos, George D. Rock, Kenneth L Mice completely lacking immunoproteasomes display major alterations in antigen presentation |
title | Mice completely lacking immunoproteasomes display major alterations in antigen presentation |
title_full | Mice completely lacking immunoproteasomes display major alterations in antigen presentation |
title_fullStr | Mice completely lacking immunoproteasomes display major alterations in antigen presentation |
title_full_unstemmed | Mice completely lacking immunoproteasomes display major alterations in antigen presentation |
title_short | Mice completely lacking immunoproteasomes display major alterations in antigen presentation |
title_sort | mice completely lacking immunoproteasomes display major alterations in antigen presentation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3262888/ https://www.ncbi.nlm.nih.gov/pubmed/22197977 http://dx.doi.org/10.1038/ni.2203 |
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