Novel Degenerate PCR Method for Whole-Genome Amplification Applied to Peru Margin (ODP Leg 201) Subsurface Samples

A degenerate polymerase chain reaction (PCR)-based method of whole-genome amplification, designed to work fluidly with 454 sequencing technology, was developed and tested for use on deep marine subsurface DNA samples. While optimized here for use with Roche 454 technology, the general framework pres...

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Detalles Bibliográficos
Autores principales: Martino, Amanda J., Rhodes, Matthew E., Biddle, Jennifer F., Brandt, Leah D., Tomsho, Lynn P., House, Christopher H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Research Foundation 2012
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3263435/
https://www.ncbi.nlm.nih.gov/pubmed/22319519
http://dx.doi.org/10.3389/fmicb.2012.00017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3263435/
https://www.ncbi.nlm.nih.gov/pubmed/22319519
http://dx.doi.org/10.3389/fmicb.2012.00017

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