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High-Resolution Whole-Mount In Situ Hybridization Using Quantum Dot Nanocrystals

The photostability and narrow emission spectra of nanometer-scale semiconductor crystallites (QDs) make them desirable candidates for whole-mount fluorescent in situ hybridization to detect mRNA transcripts in morphologically preserved intact embryos. We describe a method for direct QD labeling of m...

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Detalles Bibliográficos
Autores principales: Ioannou, Andriani, Eleftheriou, Iro, Lubatti, Andrea, Charalambous, Anna, Skourides, Paris A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3263632/
https://www.ncbi.nlm.nih.gov/pubmed/22287835
http://dx.doi.org/10.1155/2012/627602