Cargando…

High-Resolution Whole-Mount In Situ Hybridization Using Quantum Dot Nanocrystals

The photostability and narrow emission spectra of nanometer-scale semiconductor crystallites (QDs) make them desirable candidates for whole-mount fluorescent in situ hybridization to detect mRNA transcripts in morphologically preserved intact embryos. We describe a method for direct QD labeling of m...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ioannou, Andriani, Eleftheriou, Iro, Lubatti, Andrea, Charalambous, Anna, Skourides, Paris A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi Publishing Corporation 2012
Materias:	Methodology Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3263632/ https://www.ncbi.nlm.nih.gov/pubmed/22287835 http://dx.doi.org/10.1155/2012/627602

Ejemplares similares

Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo
por: Charalambous, Anna, et al.
Publicado: (2009)

A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol
por: Huang, Tian, et al.
Publicado: (2023)

Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo
por: Charalambous, Anna, et al.
Publicado: (2011)

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
por: Brend, Tim, et al.
Publicado: (2009)

An optimised whole mount in situ hybridisation protocol for the mollusc Lymnaea stagnalis
por: Hohagen, Jennifer, et al.
Publicado: (2015)

Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos
por: Gross-Thebing, Theresa, et al.
Publicado: (2014)

Protocol: an improved and universal procedure for whole-mount immunolocalization in plants
por: Pasternak, Taras, et al.
Publicado: (2015)

Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology
por: Jacobs, Nicole L., et al.
Publicado: (2011)

Protocol for RNA fluorescence in situ hybridization in mouse meningeal whole mounts
por: Nilsson, Olof Rickard, et al.
Publicado: (2022)

Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
por: Cheng, Christina N., et al.
Publicado: (2014)

Whole mount multiplexed visualization of DNA, mRNA, and protein in plant-parasitic nematodes
por: Sperling, Alexis L., et al.
Publicado: (2023)

Using modified whole-mount in situ hybridization to study mpo expression in zebrafish
por: SHEN, LI-JING, et al.
Publicado: (2013)

Electroporation-based methods for in vivo, whole mount and primary culture analysis of zebrafish brain development
por: Hendricks, Michael, et al.
Publicado: (2007)

High-Sensitivity and High-Resolution In Situ Hybridization of Coding and Long Non-coding RNAs in Vertebrate Ovaries and Testes
por: Takei, Natsumi, et al.
Publicado: (2018)

Bimodal Whole-Mount Imaging of Tendon Using Confocal Microscopy and X-ray Micro-Computed Tomography
por: Marr, Neil, et al.
Publicado: (2020)

A whole-mount in situ hybridization method for microRNA detection in Caenorhabditis elegans
por: Andachi, Yoshiki, et al.
Publicado: (2016)

Electropermeabilization-based fluorescence in situ hybridization of whole-mount plant parasitic nematode specimens
por: Ruark-Seward, Casey L., et al.
Publicado: (2019)

Lifetime blinking in nonblinking nanocrystal quantum dots
por: Galland, Christophe, et al.
Publicado: (2012)

Quantum dots: a new tool for anti-malarial drug assays
por: Ku, Min-Je, et al.
Publicado: (2011)

Modified Whole-Mount In situ Hybridization Protocol for the Detection of Transgene Expression in Electroporated Chick Embryos
por: Arede, Natacha, et al.
Publicado: (2008)

Whole-mount in situ hybridization in the Rotifer Brachionus plicatilis representing a basal branch of lophotrochozoans
por: Boell, Louis A., et al.
Publicado: (2008)

All-age whole mount in situ hybridization to reveal larval and juvenile expression patterns in zebrafish
por: Vauti, Franz, et al.
Publicado: (2020)

Contact printing of colloidal nanocrystal thin films for hybrid organic/quantum dot optoelectronic devices
por: Panzer, Matthew J., et al.
Publicado: (2012)

Application of quantum dots as vectors in targeted survivin gene siRNA delivery
por: Zhao, Jianjiang, et al.
Publicado: (2013)

Photoactive semiconductor nanocrystal quantum dots: fundamentals and applications
por: Credi, Alberto
Publicado: (2017)

Overview of Stabilizing Ligands for Biocompatible Quantum Dot Nanocrystals
por: Zhang, Yanjie, et al.
Publicado: (2011)

Quantum dot assisted tracking of the intracellular protein Cyclin E in Xenopus laevis embryos
por: Brandt, Yekaterina I, et al.
Publicado: (2015)

LNA probes substantially improve the detection of bacterial endosymbionts in whole mount of insects by fluorescent in-situ hybridization
por: Priya, Natarajan Gayatri, et al.
Publicado: (2012)

Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues
por: Lagendijk, Anne Karine, et al.
Publicado: (2012)

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
por: Hauptmann, Giselbert, et al.
Publicado: (2016)

Charge transport in semiconductors assembled from nanocrystal quantum dots
por: Yazdani, Nuri, et al.
Publicado: (2020)

Anisotropic Magnetic Resonance in Random Nanocrystal Quantum Dot Ensembles
por: Almeida, António J. S., et al.
Publicado: (2020)

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
por: Marra, Amanda N., et al.
Publicado: (2017)

Mapping Neurotransmitter Identity in the Whole-Mount Drosophila Brain Using Multiplex High-Throughput Fluorescence in Situ Hybridization
por: Meissner, Geoffrey W., et al.
Publicado: (2019)

An optimized pipeline for live imaging whole Arabidopsis leaves at cellular resolution
por: Harline, Kate, et al.
Publicado: (2023)

Target fluorescence in-situ hybridization (Target FISH) for plasma cell enrichment in myeloma
por: Ma, Edmond S. K., et al.
Publicado: (2016)

Visualization of the impatiens downy mildew pathogen using fluorescence in situ hybridization (FISH)
por: Salgado-Salazar, Catalina, et al.
Publicado: (2018)

In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections
por: Ellison, Mitchell A., et al.
Publicado: (2016)

Visualization of spatial gene expression in plants by modified RNAscope fluorescent in situ hybridization
por: Solanki, Shyam, et al.
Publicado: (2020)

An improved mounting device for attaching intracranial probes in large animal models
por: Dunster, Kimble R
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_v0re9ugrfiimlsujpus7ultvpk