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Reproducible hairy root transformation and spot-inoculation methods to study root symbioses of pea

Pea has lagged behind other model legumes in the molecular study of nodulation and mycorrhizae-formation because of the difficulty to transform its roots and its poor growth on agar plates. Here we describe for pea 1) a transformation technique which permits the complementation of two known non-nodu...

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Detalles Bibliográficos
Autores principales: Clemow, Scott R, Clairmont, Lindsey, Madsen, Lene H, Guinel, Frédérique C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3264533/
https://www.ncbi.nlm.nih.gov/pubmed/22172023
http://dx.doi.org/10.1186/1746-4811-7-46