Structural and Kinetic Evidence That Catalytic Reaction of Human UDP-glucose 6-Dehydrogenase Involves Covalent Thiohemiacetal and Thioester Enzyme Intermediates

Biosynthesis of UDP-glucuronic acid by UDP-glucose 6-dehydrogenase (UGDH) occurs through the four-electron oxidation of the UDP-glucose C6 primary alcohol in two NAD(+)-dependent steps. The catalytic reaction of UGDH is thought to involve a Cys nucleophile that promotes formation of a thiohemiacetal...

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Detalles Bibliográficos
Autores principales: Egger, Sigrid, Chaikuad, Apirat, Klimacek, Mario, Kavanagh, Kathryn L., Oppermann, Udo, Nidetzky, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2012
Materias:
Enzymology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265891/
https://www.ncbi.nlm.nih.gov/pubmed/22123821
http://dx.doi.org/10.1074/jbc.M111.313015

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3265891/
https://www.ncbi.nlm.nih.gov/pubmed/22123821
http://dx.doi.org/10.1074/jbc.M111.313015

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