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Polyamine metabolism is involved in adipogenesis of 3T3-L1 cells

Polyamines spermidine and spermine are known to be required for mammalian cell proliferation and for embryonic development. Alpha-difluoromethylornithine (DFMO), an inhibitor of ornithine decarboxylase (ODC) a limiting enzyme of polyamine biosynthesis, depleted the cellular polyamines and prevented...

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Autores principales: Ishii, Ikumi, Ikeguchi, Yoshihiko, Mano, Hiroshi, Wada, Masahiro, Pegg, Anthony E., Shirahata, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3266501/
https://www.ncbi.nlm.nih.gov/pubmed/21809076
http://dx.doi.org/10.1007/s00726-011-1037-5
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author Ishii, Ikumi
Ikeguchi, Yoshihiko
Mano, Hiroshi
Wada, Masahiro
Pegg, Anthony E.
Shirahata, Akira
author_facet Ishii, Ikumi
Ikeguchi, Yoshihiko
Mano, Hiroshi
Wada, Masahiro
Pegg, Anthony E.
Shirahata, Akira
author_sort Ishii, Ikumi
collection PubMed
description Polyamines spermidine and spermine are known to be required for mammalian cell proliferation and for embryonic development. Alpha-difluoromethylornithine (DFMO), an inhibitor of ornithine decarboxylase (ODC) a limiting enzyme of polyamine biosynthesis, depleted the cellular polyamines and prevented triglyceride accumulation and differentiation in 3T3-L1 cells. In this study, to explore the function of polyamines in adipogenesis, we examined the effect of polyamine biosynthesis inhibitors on adipocyte differentiation and lipid accumulation of 3T3-L1 cells. The spermidine synthase inhibitor trans-4-methylcyclohexylamine (MCHA) increased spermine/spermidine ratios, whereas the spermine synthase inhibitor N-(3-aminopropyl)-cyclohexylamine (APCHA) decreased the ratios in the cells. MCHA was found to decrease lipid accumulation and GPDH activity during differentiation, while APCHA increased lipid accumulation and GPDH activity indicating the enhancement of differentiation. The polyamine-acetylating enzyme, spermidine/spermine N (1)-acetyltransferase (SSAT) activity was increased within a few hours after stimulus for differentiation, and was found to be elevated by APCHA. In mature adipocytes APCHA decreased lipid accumulation while MCHA had the opposite effect. An acetylpolyamine oxidase and spermine oxidase inhibitor MDL72527 or an antioxidant N-acetylcysteine prevented the promoting effect of APCHA on adipogenesis. These results suggest that not only spermine/spermidine ratios but also polyamine catabolic enzyme activity may contribute to adipogenesis.
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spelling pubmed-32665012012-02-03 Polyamine metabolism is involved in adipogenesis of 3T3-L1 cells Ishii, Ikumi Ikeguchi, Yoshihiko Mano, Hiroshi Wada, Masahiro Pegg, Anthony E. Shirahata, Akira Amino Acids Original Article Polyamines spermidine and spermine are known to be required for mammalian cell proliferation and for embryonic development. Alpha-difluoromethylornithine (DFMO), an inhibitor of ornithine decarboxylase (ODC) a limiting enzyme of polyamine biosynthesis, depleted the cellular polyamines and prevented triglyceride accumulation and differentiation in 3T3-L1 cells. In this study, to explore the function of polyamines in adipogenesis, we examined the effect of polyamine biosynthesis inhibitors on adipocyte differentiation and lipid accumulation of 3T3-L1 cells. The spermidine synthase inhibitor trans-4-methylcyclohexylamine (MCHA) increased spermine/spermidine ratios, whereas the spermine synthase inhibitor N-(3-aminopropyl)-cyclohexylamine (APCHA) decreased the ratios in the cells. MCHA was found to decrease lipid accumulation and GPDH activity during differentiation, while APCHA increased lipid accumulation and GPDH activity indicating the enhancement of differentiation. The polyamine-acetylating enzyme, spermidine/spermine N (1)-acetyltransferase (SSAT) activity was increased within a few hours after stimulus for differentiation, and was found to be elevated by APCHA. In mature adipocytes APCHA decreased lipid accumulation while MCHA had the opposite effect. An acetylpolyamine oxidase and spermine oxidase inhibitor MDL72527 or an antioxidant N-acetylcysteine prevented the promoting effect of APCHA on adipogenesis. These results suggest that not only spermine/spermidine ratios but also polyamine catabolic enzyme activity may contribute to adipogenesis. Springer Vienna 2011-08-02 2012 /pmc/articles/PMC3266501/ /pubmed/21809076 http://dx.doi.org/10.1007/s00726-011-1037-5 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Article
Ishii, Ikumi
Ikeguchi, Yoshihiko
Mano, Hiroshi
Wada, Masahiro
Pegg, Anthony E.
Shirahata, Akira
Polyamine metabolism is involved in adipogenesis of 3T3-L1 cells
title Polyamine metabolism is involved in adipogenesis of 3T3-L1 cells
title_full Polyamine metabolism is involved in adipogenesis of 3T3-L1 cells
title_fullStr Polyamine metabolism is involved in adipogenesis of 3T3-L1 cells
title_full_unstemmed Polyamine metabolism is involved in adipogenesis of 3T3-L1 cells
title_short Polyamine metabolism is involved in adipogenesis of 3T3-L1 cells
title_sort polyamine metabolism is involved in adipogenesis of 3t3-l1 cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3266501/
https://www.ncbi.nlm.nih.gov/pubmed/21809076
http://dx.doi.org/10.1007/s00726-011-1037-5
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