Cargando…
In Vitro Assembly of Multiple DNA Fragments Using Successive Hybridization
Construction of recombinant DNA from multiple fragments is widely required in molecular biology, especially for synthetic biology purposes. Here we describe a new method, successive hybridization assembling (SHA) which can rapidly do this in a single reaction in vitro. In SHA, DNA fragments are prep...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3266897/ https://www.ncbi.nlm.nih.gov/pubmed/22291927 http://dx.doi.org/10.1371/journal.pone.0030267 |