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In Vitro Assembly of Multiple DNA Fragments Using Successive Hybridization

Construction of recombinant DNA from multiple fragments is widely required in molecular biology, especially for synthetic biology purposes. Here we describe a new method, successive hybridization assembling (SHA) which can rapidly do this in a single reaction in vitro. In SHA, DNA fragments are prep...

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Detalles Bibliográficos
Autores principales:	Jiang, Xinglin, Yang, Jianming, Zhang, Haibo, Zou, Huibin, Wang, Cong, Xian, Mo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3266897/ https://www.ncbi.nlm.nih.gov/pubmed/22291927 http://dx.doi.org/10.1371/journal.pone.0030267

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