Simple Preparation of Pacific Cod Trypsin for Enzymatic Peptide Synthesis

Trypsin from the pyloric caeca of Pacific cod (Gadus macrocephalus) was easily prepared by affinity chromatography on Benzamidine Sepharose 6B and gel filtration on Superdex 75. Pacific cod trypsin was composed of three isozymes, and their molecular masses were estimated 23,756.34 Da, 23,939.62 Da,...

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Detalles Bibliográficos
Autores principales: Fuchise, Tomoyoshi, Sekizaki, Haruo, Kishimura, Hideki, Klomklao, Sappasith, Nalinanon, Sitthipong, Benjakul, Soottawat, Chun, Byung-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3268023/
https://www.ncbi.nlm.nih.gov/pubmed/22312475
http://dx.doi.org/10.4061/2011/912382

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3268023/
https://www.ncbi.nlm.nih.gov/pubmed/22312475
http://dx.doi.org/10.4061/2011/912382

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