Expression, Immobilization and Enzymatic Properties of Glutamate Decarboxylase Fused to a Cellulose-Binding Domain

Escherichia coli-derived glutamate decarboxylase (GAD), an enzyme that catalyzes the conversion of glutamic acid to gamma-aminobutyric acid (GABA), was fused to the cellulose-binding domain (CBD) and a linker of Trichoderma harzianum endoglucanase II. To prevent proteolysis of the fusion protein, th...

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Detalles Bibliográficos
Autores principales: Park, Hyemin, Ahn, Jungoh, Lee, Juwhan, Lee, Hyeokwon, Kim, Chunsuk, Jung, Joon-Ki, Lee, Hongweon, Lee, Eun Gyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269691/
https://www.ncbi.nlm.nih.gov/pubmed/22312257
http://dx.doi.org/10.3390/ijms13010358

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269691/
https://www.ncbi.nlm.nih.gov/pubmed/22312257
http://dx.doi.org/10.3390/ijms13010358

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